linc-ror and its spliced variants 2 and 4 are significantly up-regulated in esophageal squamous cell carcinoma

نویسندگان

reza sahebi department of molecular genetics, faculty of biological sciences, tarbiat modares university, tehran, iran

mahshid malakootian cardiogenetic research center, rajaie cardiovascular medical and research center, iran university of medical sciences, tehran, iran

baharak balalaee department of molecular genetics, faculty of biological sciences, tarbiat modares university, tehran, iran

alireza shahryari stem cell research center, golestan university of medical sciences, gorgan, iran

چکیده

objective(s): similar characteristics of molecular pathways between cellular reprogramming events and tumorigenesis have been accentuated in recent years. reprogramming-related transcription factors, also known as yamanaka factors (oct4, sox2, klf4, and c-myc), are also well-known oncogenes promoting cancer initiation, progression, and cellular transformation into cancer stem cells. long non-coding rnas (lncrnas) are a major class of rna molecules with emerging roles in stem cell pluripotency, cellular reprogramming, cellular transformation, and tumorigenesis. the long intergenic non-coding rna ror (lincrna-ror, linc-ror) acts as a regulator of cellular reprograming through sponging mir-145 that normally negatively regulates the expression of the stemness factors nanog, oct4, and sox2. materials and methods: here, we employed a real-time pcr approach to determine the expression patterns of linc-ror and its two novel spliced variants (variants 2 and 4) in esophageal squamous cell carcinoma (escc). results: the quantitative real-time rt-pcr results revealed a significant up-regulation of linc-ror (p=0.0098) and its variants 2 (p=0.0250) and 4 (p=0.0002) in tumor samples of escc, compared to their matched non-tumor tissues obtained from the margin of same tumors. our data also demonstrated a significant up-regulation of variant 4 in high-grade tumor samples, in comparison to the low-grade ones (p=0.04). moreover, the roc curve analysis demonstrated that the variant 4 of ror has a potential to discriminate between tumor and non-tumor samples (auc=0.66, p<0.05). conclusion: our data suggest a significant up-regulation of linc-ror and its variants 2 and 4 in escc tissue samples.

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Linc-ROR and its spliced variants 2 and 4 are significantly up-regulated in esophageal squamous cell carcinoma

Objective(s): Similar characteristics of molecular pathways between cellular reprogramming events and tumorigenesis have been accentuated in recent years. Reprogramming-related transcription factors, also known as Yamanaka factors (OCT4, SOX2, KLF4, and c-MYC), are also well-known oncogenes promoting cancer initiation, progression, and cellular transformation into cancer stem cells. Long non-co...

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Linc-ROR and its spliced variants 2 and 4 are significantly up-regulated in esophageal squamous cell carcinoma

OBJECTIVES Similar characteristics of molecular pathways between cellular reprogramming events and tumorigenesis have been accentuated in recent years. Reprogramming-related transcription factors, also known as Yamanaka factors (OCT4, SOX2, KLF4, and c-MYC), are also well-known oncogenes promoting cancer initiation, progression, and cellular transformation into cancer stem cells. Long non-codin...

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عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۹، شماره ۱۰، صفحات ۱۱۳۱-۱۱۳۵

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